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To ensure robust results, experts recommend a sequencing depth of at least 100x, equivalent to around 12g of data This guide offers recommendations on sequencing coverage, depth and numbers of reads for genomic and transciptomic based applications. It depends on what the goals are
Danielle For Aurora
30x is generally considered sufficient for most research purposes For example, when you get 30x wgs, the '30x' means that your entire genome will be sequenced an average of 30 times. 100x or higher is typical for clinical applications
Some factors i consider in no particular order:
Nebula genomics is the best brand for whole genome sequencing according to lifespan society of bc, with up to 100x sequencing depth allowing for highly accurate dna sequencing data. There is no general guideline for determining the optimal coverage for a sequencing project It highly depends on the type of experiment, the species, the input material, the sequencing platform and other factors. Why do you think you need to be 100x full genome sequenced
You'll find you have a ton of rare variants, like everyone does, and you'll be left knowing nothing you didn't know before Common variants could be found by 23andme or similar. Based on reading around, it seems that 100x genome sequencing is best for clinical situations and rare things, along with issues in the mitochondria Would this be correct, or would i be better off going with the 30x sequencing
What benefit will 100x give me over the 30x?
When you see genome sequencing being sold, 'x' marks the spot to look for The number before the 'x' is the coverage (the average number of times your genome will be sequenced)
