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Innovations in linker design are focused on multiple issues ranging from serum stability to mechanism of release to drug to antibody ratio (dar) One of the main challenges in developing a safe and effective adc drug (figure 1) is the assembly of a desirable chemical linker between cytotoxic payload and mab As linkers become increasingly sophisticated, more emphasis is being placed on the methods of bioconjugation between linker and antibody, with the goal of producing homogeneous adc populations.

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An antibody is linked to a small molecule via a covalent tether Role of adc linkers adc linkers play key roles in determining the overall success of the antibody drug conjugates Target, antibody, linker, payload, and conjugation method.

Linker tethering payload to antibody is vital for adc

This review provides an overview of linkers of 17 approved adcs and recent innovations in linker design, focusing on drug release triggers, bioconjugation strategies, the impact of spacers on hydrophilicity, traceless drug release, and linker architecture, as well as a discussion of the bystander effect, offering insights for the rational. An ideal linker should be stable in the circulatory system and release the cytotoxic payload specifically in the tumor